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| 探針法做為microRNA定量研究的金標(biāo)準(zhǔn),其具有高特異性、高靈敏度的優(yōu)勢。HaiGene的HG TaqMan miRNA定量PCR試劑盒,采用特異性的正向miRNA引物和接頭引物進(jìn)行PCR擴(kuò)增,檢測熒光基團(tuán)采用FAM標(biāo)記的TaqMan探針。使用該試劑盒可以高特異性、高靈敏度的檢測低至100個(gè)細(xì)胞的miRNA分子,并可區(qū)分相似度極高的miRNAs。HaiGene的TaqMan miRNA定量PCR試劑盒多達(dá)一萬余種,試劑盒編號為TAPxxxxx,每一個(gè)mircoRNA分子對應(yīng)一個(gè)檢測試劑盒(包含特異性的TaqMan探針、正向引物、反向引物、定量試劑),可于HG TaqMan miRNA qPCR kit.xls中查詢。 特別說明:如您研究的miRNA不在我們的TaqMan miRNA qPCR Kit列表之中,請登錄www.mirbase.org進(jìn)行miRNA查詢,并來信告知Accession No.和序列,我們將在2周內(nèi)完成試劑盒的定制。 |
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原理 |
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組分 |
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儲存 |
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| 避光置于-20°C,可保存2年;避免反復(fù)凍融。 | |||||||||||||||
檢測效果和應(yīng)用實(shí)例 |
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| 注:其它miRNA熒光定量檢測試劑盒請查詢HG TaqMan miRNA qPCR kit.xls。 | |||||||||||||||
參考文獻(xiàn) |
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| [1] Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis. Mol Biol Rep. 2013. PMID: 23307300. [2] Quantitative stem-loop RT-PCR for detection of microRNAs. Methods Mol Biol. 2011. PMID: 21533691. [3] MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). J Vis Exp. 2012. PMID: 22643910. [4] Circulating microRNA-196a as a candidate diagnostic biomarker for chronic hepatitis C. Mol Med Rep. 2015. PMID: 25738504. [5] Novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity. PLoS One. 2012. PMID: 23152780. [6] Diagnostic microRNA markers to screen for sporadic human colon cancer in stool: I. Proof of principle. Cancer Genomics Proteomics. 2013. PMID: 23741026. [7] Stem-loop RT-qPCR for microRNA expression profiling. Methods Mol Biol. 2012. PMID: 22144190. [8] Serum microRNA-21 as marker for necroinflammation in hepatitis C patients with and without hepatocellular carcinoma. PLoS One. 2011. PMID: 22066022. |
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